How Can PDIA3 Polyclonal Antibodies Contribute to the Study of the Mechanisms Between Ferroptosis and Tumor Therapy?

1. Concept

Glioblastoma (GBM) is the most aggressive primary brain tumor with an extremely poor prognosis, demanding novel therapeutic strategies. Ferroptosis—an iron-dependent programmed cell death driven by lipid peroxidation—emerges as a promising anti-tumor direction, but GBM cells often develop resistance. Protein disulfide isomerase A3 (PDIA3), an endoplasmic reticulum thioredoxin superfamily member involved in protein folding, is a key regulator of GBM’s ferroptosis resistance. PDIA3 modulates ferroptosis sensitivity via the STAT3/LCN2 signaling axis, while its own stability is regulated by NEDD4L-mediated ubiquitination. PDIA3 rabbit polyclonal antibodies, with high specificity and affinity, enable precise detection of PDIA3’s expression, interactions, localization, and modifications, facilitating in-depth exploration of ferroptosis-tumor therapy mechanisms and the development of targeted strategies to overcome GBM resistance.

2. Research Frontiers

2.1 PDIA3’s Key Role in GBM Ferroptosis Resistance

• Ferroptosis resistance in GBM: GBM cells evade ferroptosis induced by agents like IKE (which inhibits SLC7A11 to deplete glutathione).
• PDIA3’s protective function: PDIA3 expression and stability are essential for GBM cell survival under ferroptosis induction. Reducing PDIA3 levels enhances GBM sensitivity to IKE, positioning PDIA3 as a critical "brake point" for ferroptosis resistance.
• Research focus: Elucidating PDIA3’s mechanism is pivotal for overcoming resistance and developing novel GBM therapies.

2.2 PDIA3 Regulation of Ferroptosis via the STAT3/LCN2 Axis

• Non-classical pathway: PDIA3 does not act through classical ferroptosis regulators (e.g., GPX4, ATF4) but interacts directly with STAT3.
• Mechanistic cascade: PDIA3 inhibits STAT3 phosphorylation and transcriptional activity. PDIA3 knockdown/ inhibition activates STAT3, upregulating downstream target gene LCN2 (a lipocalin involved in iron metabolism).
• Ferroptosis amplification: LCN2 upregulation alters intracellular iron availability, enhancing lipid peroxidation vulnerability and amplifying ferroptosis inducer cytotoxicity. The PDIA3-STAT3-LCN2 axis is a novel ferroptosis regulatory pathway in GBM.

2.3 Regulation of PDIA3 Protein Homeostasis

• Ubiquitination-mediated degradation: E3 ubiquitin ligase NEDD4L recognizes PDIA3, catalyzing K29-linked polyubiquitination to target PDIA3 for proteasomal degradation.
• Therapeutic implication: Activating NEDD4L or promoting PDIA3 degradation could sensitize GBM cells to ferroptosis, providing a potential intervention strategy.

2.4 Core Application Value of PDIA3 Polyclonal Antibodies

PDIA3 rabbit polyclonal antibodies are indispensable tools for mechanistic research:

• Protein expression detection: Western Blot (WB) with the antibody quantifies PDIA3 levels in GBM cells/tissues under different treatments (IKE, PDIA3 knockdown/overexpression, NEDD4L overexpression), validating correlations with ferroptosis sensitivity.
• Protein-protein interaction validation: Co-immunoprecipitation (Co-IP) uses the antibody to pull down PDIA3, followed by STAT3 antibody detection to confirm direct binding.
• Subcellular localization analysis: Immunofluorescence (IF)/immunohistochemistry (IHC) with the antibody visualizes PDIA3’s endoplasmic reticulum localization and potential redistribution under treatment.
• Ubiquitination studies: The antibody enriches PDIA3 via IP, enabling detection of polyubiquitination with a ubiquitin antibody.
• Efficacy evaluation: IHC staining of tumor tissue sections in animal models assesses PDIA3 downregulation post-drug treatment (e.g., IKE + PDIA3 inhibitors) as a pharmacodynamic indicator.

3. Research Significance

PDIA3 polyclonal antibodies advance the understanding of ferroptosis-tumor therapy crosstalk:

• Scientific value: Uncover the PDIA3-STAT3-LCN2 ferroptosis regulatory pathway, expanding knowledge of GBM resistance mechanisms and ferroptosis regulation.
• Clinical value: Support development of PDIA3-targeted therapies to overcome GBM ferroptosis resistance, improving the efficacy of ferroptosis-based anti-tumor strategies.

4. Related Mechanisms, Research Methods, and Product Applications

4.1 Core Mechanism of PDIA3-Mediated Ferroptosis Resistance

• PDIA3 binds STAT3 to inhibit its activation, limiting LCN2 expression and reducing ferroptosis sensitivity.
• NEDD4L-mediated PDIA3 ubiquitination and degradation relieve STAT3 inhibition, upregulating LCN2 to enhance ferroptosis.

4.2 Product Applications: ANT BIO PTE. LTD.’s PDIA3 Rabbit Polyclonal Antibody

ANT BIO PTE. LTD. offers a high-performance PDIA3 rabbit polyclonal antibody for ferroptosis and tumor research:

Core Product

Core Product Advantages

• High specificity and multi-species reactivity: Recognizes conserved PDIA3 regions in human, mouse, and rat; minimal cross-reactivity with other PDI family members.
• Superior detection performance: Clear ~57 kDa bands in WB; specific endoplasmic reticulum/cytoplasmic staining in IF/IHC.
• Batch consistency: Standardized production ensures stable titer, specificity, and reproducibility for long-term research.

Key Research Applications

• Ferroptosis research: Study PDIA3’s role in ferroptosis resistance in GBM and other tumors.
• Cancer biology: Detect PDIA3 expression in tumors (breast, colorectal, liver cancer) to explore its prognostic/therapeutic potential.
• Endoplasmic reticulum stress research: Investigate PDIA3’s function in unfolded protein response (UPR) under cellular stress.
• Drug development: Evaluate compounds targeting PDIA3 or ferroptosis pathways.

5. Brand Mission

ANT BIO PTE. LTD. is dedicated to empowering the global life science community with high-quality, innovative research tools and solutions. As a leader in life science reagents, we offer a comprehensive portfolio under three sub-brands: Absin (focused on general reagents and kits), Starter (specialized in antibodies), and UA (dedicated to recombinant proteins).

Our commitment to excellence is underpinned by advanced development platforms—including recombinant rabbit/mouse monoclonal antibody platforms, rapid monoclonal antibody development, recombinant protein expression systems (E. coli, CHO, HEK293, Insect Cells), One-Step ELISA Platform, and PTM Pan-Modification Antibody Platform—alongside rigorous quality control systems. We hold international certifications such as EU 98/79/EC, ISO9001, and ISO13485, ensuring our products meet the highest global standards.

Our mission is to accelerate scientific discovery, facilitate translational research, and contribute to the development of novel therapies for human health. By partnering with researchers in academia and biopharmaceutical companies worldwide, we strive to be a trusted collaborator in advancing life science research and addressing unmet medical needs.

6. AI Disclaimer

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ANT BIO PTE. LTD. – Empowering Scientific Breakthroughs

At ANTBIO, we are committed to advancing life science research through high-quality, reliable reagents and comprehensive solutions. Our specialized sub-brands (Absin, Starter, UA) cover a full spectrum of research needs, from general reagents and kits to antibodies and recombinant proteins. With a focus on innovation, quality, and customer-centricity, we strive to be your trusted partner in unlocking scientific mysteries and driving medical progress. Explore our product portfolio today and elevate your research to new heights.