WB result of CGA/HCG-α Rabbit mAb
Primary antibody: CGA/HCG-α Rabbit mAb at 1/1000 dilution
Lane 1: JAR whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 13 kDa
Observed MW: 18 kDa
(This blot was developed with high sensitivity substrate)
S-RMab® CGA/HCG-α Recombinant Rabbit mAb (SDT-643-61)
S-RMab® CGA/HCG-α Recombinant Rabbit mAb (SDT-643-61)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CGA/HCG-α |
| Synonyms | Glycoprotein hormones alpha chain, CG-alpha, FSH-alpha, LSH-alpha, TSH-alpha |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P01215 |
| Clone Number | SDT-643-61 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:500 | null |
Background
Human chorionic gonadotropin (HCG) is a glycoprotein hormone consisting of two noncovalently bonded subunits, alpha and beta [PMID: 68892], and is normally synthesized by placental trophoblasts during pregnancy. The alpha subunit of hCG is identical to the alpha subunit of 3 hormones synthesized by the anterior pituitary gland but the beta subunit of each of these hormones is unique and confers biological specificity. CGA is mainly used for the diagnosis and adjuvant diagnosis of placental trophoblast and germ cell tumors such as hyperemesis gravidarum and choriocarcinoma.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human placenta. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human spleen. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human testis. Anti-CGA/HCG-α antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
