IL-10 Protein, Mouse
IL-10 Protein, Mouse
Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | Interleukin-10,CSIF |
| Accession | P18893 |
| Amino Acid Sequence | Ser19-Ser178 |
| Expression System | E.coli |
| Molecular Weight | Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids. |
| Purity | ＞96% by SDS-PAGE or HPLC. |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | 20 mM PB, with 150 mM NaCl, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2) . The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Rα/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature murine IL-10 shares 70%-77% amino acid sequence identity with human, bovine, porcine IL-10, but murine IL-10 does not act on human cells. Recombinant murine IL-10 is an 18.7kDa protein containing 160 amino acid residues.
