WB result of YB1 Recombinant Rabbit mAb
Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: SW480 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SK-BR-3 whole cell lysate 20 µg
Lane 5: T-47D whole cell lysate 20 µg
Lane 6: Jurkat whole cell lysate 20 µg
Lane 7: A549 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 47 kDa
YB1 Recombinant Rabbit mAb (S-1748-144)
YB1 Recombinant Rabbit mAb (S-1748-144)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | YB1 |
| Synonyms | Y-box-binding protein 1; YB-1; CCAAT-binding transcription factor I subunit A (CBF-A); DNA-binding protein B (DBPB); Enhancer factor I subunit A (EFI-A); Nuclease-sensitive element-binding protein 1; Y-box transcription factor; NSEP1; YBX1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Secreted |
| Accession | P67809 |
| Clone Number | S-1748-144 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, SW480, MCF7, SK-BR-3, T-47D, Jurkat, A549, NIH/3T3, C6 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Y-box-binding protein 1 (YB-1), encoded by YBX1, is a multifunctional nucleic-acid-binding member of the cold-shock domain family that shuttles between cytoplasm and nucleus (the latter upon stress); it acts as a transcription factor, co-activator or repressor by binding the Y-box (5′-CTGATTGG-3′) and other elements, regulates mRNA splicing, stability and translation by associating with mRNPs and modulating cap-dependent/IRES-driven initiation, participates in DNA repair and stress responses, and its overexpression and nuclear accumulation are linked to cancer proliferation, metastasis, multidrug resistance (via MDR1, EGFR, CCNA/B1, etc.) and poor prognosis, making it a diagnostic marker and therapeutic target.
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Picture
Western Blot
WB result of YB1 Recombinant Rabbit mAb
Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 45 kDa
WB result of YB1 Recombinant Rabbit mAb
Primary antibody: YB1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 36 kDa
Observed MW: 45 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostatic cancer. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stoamch. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-YB1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- YB1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
