WB result of TNF alpha Recombinant Rabbit mAb
Primary antibody: TNF alpha Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated THP-1 whole cell lysate 20 µg
Lane 2: THP-1 treated with 80 nM TPA and 1 ug/ml LPS overnight, then add 500 ng/ml BFA for 6 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 23 kDa
TNF alpha Recombinant Rabbit mAb (S-2506-69)
TNF alpha Recombinant Rabbit mAb (S-2506-69)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TNF alpha |
| Synonyms | Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2 (TNF-a); TNFA; TNFSF2; TNF |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Secreted |
| Accession | P01375 |
| Clone Number | S-2506-69 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
| ICC | 1:500 | Hu |
Background
Tumor necrosis factor alpha (TNF-α) is a pleiotropic, 17-kDa homotrimeric cytokine synthesized chiefly by activated macrophages but also by T cells, NK cells, fibroblasts, and adipocytes; it signals through two distinct receptors, TNFR1 and TNFR2, to orchestrate a vast array of biological processes including activation of NF-κB and MAPK pathways that drive pro-inflammatory gene expression, induction of fever, chemokine secretion for leukocyte recruitment, enhancement of cytotoxic CD8+ T cell responses, and promotion of apoptosis or necroptosis via caspase-8 or RIPK1/RIPK3 signaling, while also mediating cachexia and insulin resistance in chronic inflammation, and its dysregulated overproduction is a central driver of rheumatoid arthritis, inflammatory bowel disease, psoriasis, sepsis, and neurodegeneration, making it a prime therapeutic target for monoclonal antibodies such as infliximab, adalimumab, and certolizumab pegol.
Picture
Picture
Western Blot
Immunocytochemistry
ICC analysis of THP-1 cells treated with TPA (80nM, overnight) and LPS (1ug/ml, overnight) and BFA (500ng/ml, last 6 hrs) (top panel) and untreated THP-1 cells (below panel). Anti- TNF alpha antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
