WB result of TGF beta 1 Recombinant Rabbit mAb
Primary antibody: TGF beta 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: A549 whole cell lysate 20 µg
Lane 3: HL-60 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 13, 54 kDa
This blot was developed with high sensitivity substrate
TGF beta 1 Recombinant Rabbit mAb (S-3204)
TGF beta 1 Recombinant Rabbit mAb (S-3204)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TGF beta 1 |
| Synonyms | Transforming growth factor beta-1 proprotein; TGFB; TGFB1 |
| Location | Secreted |
| Accession | P01137 |
| Clone Number | S-3204 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, A549, HL-60, SH-SY5Y, NIH/3T3, RAW264.7, mouse spleen, C6, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
Transforming Growth Factor-beta 1 (TGF-β1) is a multifunctional 25 kDa homodimeric cytokine that belongs to the TGF-β superfamily and is secreted by virtually all somatic cells as a large latent complex bound to latency-associated peptide and latent TGF-β binding protein; upon activation by proteases or integrins it signals through a heteromeric receptor complex of TβRII and ALK5 (TβRI) serine/threonine kinases to phosphorylate Smad2/3, which then complex with Smad4 to regulate transcription of hundreds of genes that orchestrate pleiotropic effects ranging from embryogenesis, extracellular matrix deposition, immune suppression and wound healing to cell cycle arrest, apoptosis, and epithelial-mesenchymal transition, with dysregulation implicated in fibrosis, cancer, and autoimmune disease.
Picture
Picture
Western Blot
WB result of TGF beta 1 Recombinant Rabbit mAb
Primary antibody: TGF beta 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Lane 3: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 13, 54 kDa
This blot was developed with high sensitivity substrate
WB result of TGF beta 1 Recombinant Rabbit mAb
Primary antibody: TGF beta 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 44 kDa
Observed MW: 13, 54 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human bone marrow. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bone marrow. Anti-TGF beta 1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
