WB result of Stat5 Recombinant Rabbit mAb
Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: HDLM-2 whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Lane 6: Jurkat whole cell lysate 20 µg
Negative control: PC-3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
Stat5 Recombinant Rabbit mAb (S-2823-24)
Stat5 Recombinant Rabbit mAb (S-2823-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | STAT5 |
| Synonyms | Signal transducer and activator of transcription 5B; STAT5B |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P51692 |
| Clone Number | S-2823-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | K562, HDLM-2, Raji, HeLa, Jurkat, A20, mouse heart, PC-12, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:50-1:200 | Hu, Ms, Rt |
Background
Stat5 (Signal Transducer and Activator of Transcription 5) is a latent cytoplasmic transcription factor that becomes phosphorylated on a conserved tyrosine (Tyr694 in human STAT5a) by activated Janus kinases—primarily JAK2—following cytokine (e.g., IL-2, IL-3, GM-CSF, GH, PRL, EPO, TPO) or oncogenic tyrosine-kinase receptor (e.g., BCR-ABL, FLT3-ITD, JAK2V617F) signaling, whereupon it forms parallel homo- or heterodimers, translocates into the nucleus, binds the consensus DNA motif TTCNNNGAA within enhancers/promoters of target genes like CISH, BCL2, MYC, CCND1, PIM1 and SOCS proteins, and thereby orchestrates proliferation, survival, self-renewal and differentiation programs in hematopoietic, mammary and other cell types; persistent Stat5 activation is a driver of myeloproliferative neoplasms, acute leukemias and breast cancers, making its upstream kinases and the Stat5 SH2 or DNA-binding domains attractive therapeutic targets.
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Western Blot
WB result of Stat5 Recombinant Rabbit mAb
Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse heart lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
WB result of Stat5 Recombinant Rabbit mAb
Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
WB result of Stat5 Recombinant Rabbit mAb
Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 90 kDa
WB result of Stat5 Recombinant Rabbit mAb
Primary antibody: Stat5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Human stat5a full length recombinant protein 10 ng
Lane 2: Human stat5b full length recombinant protein 10 ng
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 90 kDa
Observed MW: 100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-stat5 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti-stat5 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti-stat5 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
