WB result of SEMA5A Recombinant Rabbit mAb
Primary antibody: SEMA5A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: U-87 MG whole cell lysate 20 µg
Lane 3: HaCaT whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 130 kDa
This blot was developed with high sensitivity substrate
SEMA5A Recombinant Rabbit mAb (S-2560-24)
SEMA5A Recombinant Rabbit mAb (S-2560-24)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SEMA5A |
| Synonyms | Semaphorin-5A; Semaphorin-F (Sema F); SEMAF |
| Immunogen | Synthetic Peptide |
| Location | Membrane |
| Accession | Q13591 |
| Clone Number | S-2560-24 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | A549, U-87 MG, HaCaT, A431, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms, Rt |
Background
SEMA5A (Semaphorin-5A) is a transmembrane glycoprotein encoded by the SEMA5A gene that belongs to the class 5 semaphorin subfamily, featuring an extracellular sema domain, seven thrombospondin type-1 repeats (TSR1-7), and a short cytoplasmic tail. Originally identified as an axon guidance cue, SEMA5A is bifunctional, either promoting or inhibiting axon growth and cell migration depending on context, and it binds plexin-B3 to regulate neural circuit development in the retina, hippocampus, cortex, and spinal cord. Beyond the nervous system, SEMA5A exhibits context-dependent roles in tumorigenesis (e.g., promoting pancreatic cancer invasion but suppressing lung adenocarcinoma proliferation and migration) and in immune modulation (e.g., driving inflammation in rheumatoid arthritis and chronic urticaria). Importantly, dysregulation or mutation of SEMA5A is genetically linked to neurodevelopmental disorders such as autism spectrum disorder, intellectual disability, and Parkinson’s disease, with reduced expression observed in autistic brains.
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Western Blot
WB result of SEMA5A Recombinant Rabbit mAb
Primary antibody: SEMA5A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 125 kDa
This blot was developed with high sensitivity substrate
WB result of SEMA5A Recombinant Rabbit mAb
Primary antibody: SEMA5A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 121 kDa
Observed MW: 125 kDa
This blot was developed with high sensitivity substrate
