WB result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Rabbit mAb
Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: 293T whole cell lysate 20 µg
Lane 3: phosphatase treated HeLa whole cell lysate 20 µg
Lane 4: phosphatase treated 293T whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 217 kDa
Observed MW: 290 kDa
RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Rabbit mAb (S-2410-79)
RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Rabbit mAb (S-2410-79)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | RNA polymerase II CTD repeat YSPTSPS (phospho S5) |
| Synonyms | DNA-directed RNA polymerase II subunit RPB1; RNA polymerase II subunit B1; 3'-5' exoribonuclease; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase III largest subunit; RNA-directed RNA polymerase II subunit RPB1; POLR2; POLR2A |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P24928 |
| Clone Number | S-2410-79 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, 293T |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
RNA polymerase II CTD repeat YSPTSPS (phospho S5) is a crucial component of the RNA polymerase II complex, specifically referring to the phosphorylated serine 5 residue within the heptapeptide repeat sequence YSPTSPS in the CTD (C-terminal domain) of the largest subunit of RNA polymerase II. This phosphorylation of serine 5 plays a significant role in the transcription process, particularly during the initiation and early elongation phases. It helps in the recruitment of transcription factors and other regulatory proteins, facilitating the assembly of the pre-initiation complex and enabling efficient transcription initiation. Additionally, the phosphorylation status of S5 can influence the interaction of RNA polymerase II with other components of the transcription machinery, thereby regulating gene expression and ensuring proper processing of nascent RNA transcripts.
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Picture
Western Blot
Dot Blot
Dot blot result of RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Rabbit mAb
Lane 1: RNA polymerase ll CTD repeat YSPTSPS (Ser5) phospho peptide
Lane 2: RNA polymerase ll CTD repeat YSPTSPS (Tyr1) phospho peptide
Lane 3: RNA polymerase ll CTD repeat YSPTSPS (Ser2) phospho peptide
Lane 4: RNA polymerase ll CTD repeat YSPTSPS (Thr4) phospho peptide
Lane 5: RNA polymerase ll CTD repeat YSPTSPS (Ser7) phospho peptide
Lane 6: RNA polymerase ll CTD repeat YSPTSPS unmodified peptide
Primary antibody: RNA polymerase II CTD repeat YSPTSPS (phospho S5) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Immunocytochemistry
ICC analysis of HeLa cells treated with alkaline phosphatase for 1 hour (top panel) and untreated HeLa cells (below panel). Anti- Phospho-RNA polymerase II CTD repeat YSPTSPS (S5) antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
