WB result of NUP98 Recombinant Rat mAb
Primary antibody: NUP98 Recombinant Rat mAbat 1/1000 dilution
Lane 1: HeLa nuclear lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 98 kDa
NUP98 Recombinant Rat mAb (S-3384)
NUP98 Recombinant Rat mAb (S-3384)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | NUP98 |
| Synonyms | Nuclear pore complex protein Nup98-Nup96; 98 kDa nucleoporin; Nucleoporin Nup98 (Nup98); Nuclear pore complex protein Nup96 Alternative names: 96 kDa nucleoporin; Nucleoporin Nup96 (Nup96); ADAR2 |
| Location | Nucleus |
| Accession | P52948 |
| Clone Number | S-3384 |
| Antibody Type | Rat mAb |
| Isotype | IgG2c |
| Application | WB |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, SH-SY5Y, NIH/3T3, RAW264.7, PC-12, C6, COS-7 |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
Background
NUP98 (nucleoporin 98) is a 98-kDa component of the nuclear pore complex (NPC) generated by autoproteolytic cleavage of a 186-kDa precursor that also yields NUP96 . Anchored on the nucleoplasmic face of the NPC, it contains N-terminal GLFG/FG repeats that serve as docking sites for transport receptors during nucleocytoplasmic trafficking of proteins and RNAs, while its C-terminal RNA-binding domain participates in mRNA export and gene-expression regulation . Beyond transport, NUP98 dynamically associates with mitotic structures to control spindle assembly and mitotic progression, forms liquid-droplet compartments that sequester damaged DNA to safeguard genome integrity, and acts as a transcriptional coactivator or corepressor by localizing to developmental gene loci. Chromosomal translocations that fuse the N-terminal portion of NUP98 to over 30 partner proteins (e.g., homeodomain transcription factors) generate potent oncoproteins that drive aggressive forms of acute myeloid leukemia and T-cell acute lymphoblastic leukemia, highlighting its pivotal role in both normal cellular physiology and oncogenesis.
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Western Blot
WB result of NUP98 Recombinant Rat mAb
Primary antibody: NUP98 Recombinant Rat mAbat 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 98 kDa
WB result of NUP98 Recombinant Rat mAb
Primary antibody: NUP98 Recombinant Rat mAbat 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 98 kDa
WB result of NUP98 Recombinant Rat mAb
Primary antibody: NUP98 Recombinant Rat mAbat 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 98 kDa
Observed MW: 98 kDa
