WB result of NF-kB p105/p50 Recombinant Rabbit mAb
Primary antibody: NF-kB p105/p50 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: THP-1 whole cell lysate 20 µg
Lane 4: Raji whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Lane 6: Daudi whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 50, 105 kDa
NF-κB p105/p50 Recombinant Rabbit mAb (S-3354)
NF-κB p105/p50 Recombinant Rabbit mAb (S-3354)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | NF-κB p105/p50 |
| Synonyms | Nuclear factor NF-kappa-B p105 subunit; DNA-binding factor KBF1; EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; NFKB1 |
| Location | Cytoplasm, Nucleus |
| Accession | P19838 |
| Clone Number | S-3354 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Jurkat, THP-1, Raji, MCF7, Daudi, A20, mouse spleen, rat spleen |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu |
Background
The NF-κB p105 precursor protein is a 105 kDa cytoplasmic ankyrin-repeat-rich molecule that undergoes stimulus-dependent, ubiquitin/proteasome-mediated processing to liberate the 50 kDa DNA-binding p50 subunit, which then dimerizes with other NF-κB/Rel members to form transcriptionally active complexes that rapidly enter the nucleus and regulate genes governing immunity, inflammation, cell survival, and proliferation; p105 itself also serves as an IκB-like inhibitor by sequestering associated dimers in the cytoplasm, and its balanced processing versus full-length stability is tightly controlled by IKK-mediated phosphorylation, ensuring dynamic NF-κB signaling in response to cytokines, pathogens, and cellular stresses.
Picture
Picture
Western Blot
WB result of NF-kB p105/p50 Recombinant Rabbit mAb
Primary antibody: NF-kB p105/p50 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A20 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 50, 105 kDa
WB result of NF-kB p105/p50 Recombinant Rabbit mAb
Primary antibody: NF-kB p105/p50 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 105 kDa
Observed MW: 50, 105 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human tonsil. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti- NF-kB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti- NF-κB p105/p50 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
