WB result of Insulin Receptor β Recombinant Rabbit mAb
Primary antibody: Insulin Receptor β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: HEK-293 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HCT 116 whole cell lysate 20 µg
Lane 5: MCF7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 156 kDa
Observed MW: 105 kDa
This blot was developed with high sensitivity substrate
Insulin Receptor β Recombinant Rabbit mAb (S-2605-10)
Insulin Receptor β Recombinant Rabbit mAb (S-2605-10)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Insulin Receptor β |
| Synonyms | Insulin receptor; IR; CD220; INSR |
| Immunogen | Synthetic Peptide |
| Location | Lysosome, Endosome, Cell membrane |
| Accession | P06213 |
| Clone Number | S-2605-10 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu, Ms |
| Positive Sample | HepG2, HEK-293, HeLa, HCT 116, MCF7, NIH/3T3 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu, Ms |
Background
The insulin receptor β-subunit is the 95 kDa transmembrane tyrosine kinase moiety of the heterotetrameric insulin receptor, spanning the plasma membrane once with its short extracellular domain stabilizing disulfide linkage to the α-subunit and its large cytoplasmic tail harboring an ATP-binding site and catalytic domain that, upon insulin-induced autophosphorylation of specific tyrosines such as Y1158, Y1162 and Y1163, recruits and phosphorylates IRS and Shc adaptor proteins to initiate PI3K-Akt and MAPK signaling cascades governing glucose uptake, glycogen synthesis, lipogenesis and cell growth, while mutations in the β-subunit that impair kinase activity or endocytosis are linked to severe insulin resistance syndromes including Rabson–Mendenhall and type A diabetes.
Picture
Picture
Western Blot
WB result of Insulin Receptor β Recombinant Rabbit mAb
Primary antibody: Insulin Receptor β Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 156 kDa
Observed MW: 105 kDa
This blot was developed with high sensitivity substrate
