ICC shows positive staining in Histone H3-GFP transfected 293F cells (top panel) and negative staining in vector-transfected 293F cells (below panel). Anti- GFP (Alexa Fluor® 488 Conjugate) antibody was used at 1/50 dilution (Green) and incubated overnight at 4°C. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).
GFP Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-296-32)
GFP Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (S-296-32)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | GFP |
| Synonyms | Green fluorescent protein |
| Immunogen | Synthetic Peptide |
| Accession | P42212 |
| Clone Number | S-296-32 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ICC |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:50 | Species Independent |
Background
The green fluorescent protein (GFP) is a ~27 kDa protein composed of 238 amino acids, originally isolated from the jellyfish Aequorea victoria. Its core structure features a unique β-barrel fold with a central fluorescent chromophore (formed by the autocatalytic cyclization and oxidation of Ser65-Tyr66-Gly67). When excited by blue light at 395 nm (primary peak) or 475 nm (secondary peak), GFP emits green fluorescence at 509 nm without requiring additional cofactors. This property has made GFP a revolutionary tool in molecular and cellular biology, widely used for gene expression reporting, protein localization tracking, and dynamic cell imaging.
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Immunocytochemistry
