WB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HepG2 whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 35 kDa
Fibrillarin Recombinant Rabbit mAb (S-1718-171)
Fibrillarin Recombinant Rabbit mAb (S-1718-171)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Fibrillarin |
| Synonyms | rRNA 2'-O-methyltransferase fibrillarin; 34 kDa nucleolar scleroderma antigen; Histone-glutamine methyltransferase; U6 snRNA 2'-O-methyltransferase fibrillarin; FIB1; FLRN; FBL |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P22087 |
| Clone Number | S-1718-171 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, HeLa, HepG2, Molt-4, Jurkat, NIH/3T3, mouse liver, C6, rat testis, COS-7 |
| Predicted Reactivity | Dr, Xe, Fu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:50-1:100 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
Fibrillarin is an evolutionarily conserved, 34-kDa nucleolar methyltransferase that is essential for 2'-O-methylation of pre-ribosomal RNA and pre-assembly of small nucleolar ribonucleoprotein (snoRNP) complexes on nascent ribosomal RNA, thereby driving early steps of ribosome biogenesis; it contains an N-terminal glycine- and arginine-rich (GAR) domain that mediates nucleolar localization and interaction with box C/D snoRNAs, followed by a central RNA-binding domain and a C-terminal S-adenosyl-L-methionine-dependent methyltransferase motif, localizes predominantly to the dense fibrillar component of the nucleolus in an Nop56/Nop58- and 15.5 kDa protein-dependent manner, and is recruited to sites of rDNA transcription by UBF and SL1; mutations or depletion of fibrillarin impair rRNA processing, leading to defects in 18S and 28S rRNA maturation, nucleolar stress, cell-cycle arrest, and embryonic lethality in model organisms, while in humans its overexpression is linked to several cancers and its absence causes the neurodevelopmental disorder Néstor-Guillermo progeria syndrome, underscoring its dual role as a guardian of ribosome production and a sensor of nucleolar integrity.
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Western Blot
WB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
WB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat testis lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
WB result of Fibrillarin Recombinant Rabbit mAb
Primary antibody: Fibrillarin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 34 kDa
Observed MW: 36 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse liver. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Fibrillarin antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-Fibrillarin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
