WB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDa
ELP3 Rabbit Polyclonal Antibody
ELP3 Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ELP3 |
| Synonyms | Elongator complex protein 3; hELP3; tRNA uridine(34) acetyltransferase |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | Q9H9T3 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HeLa, MCF7, K562, Jurkat, Neuro-2a, C6, COS-7 |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:100 | Hu, Ms |
Background
ELP3 (Elongator complex protein 3, also known as KAT9) is the catalytic histone acetyltransferase subunit of the highly conserved Elongator complex (composed of ELP1–ELP6) that associates with RNA polymerase II to facilitate transcriptional elongation via acetylation of histones H3 and H4, while also exhibiting cytoplasmic roles in α-tubulin acetylation to regulate neuronal migration and axonal branching, modifying wobble uridines in tRNAs to enhance translational efficiency, and participating in DNA demethylation after fertilization, cell migration, and redox homeostasis through acetylation of glucose-6-phosphate dehydrogenase, with its dysfunction implicated in neurodevelopmental disorders and tumor progression.
Picture
Picture
Western Blot
WB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDa
This blot was developed with high sensitivity substrate
WB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDa
WB result of ELP3 Rabbit pAb
Primary antibody: ELP3 Rabbit pAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 62 kDa
Observed MW: 55 kDa
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- ELP3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
ICC shows positive staining in Neuro-2a cells. Anti- ELP3 antibody was used at 1/100 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
