WB result of CXCL1 Recombinant Rabbit mAb
Primary antibody: CXCL1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: untreated RAW264.7 whole cell lysate 20 µg
Lane 2: RAW264.7 treated with 100 ng/ml LPS for 6 hours, then add 300 ng/ml Brefeldin A for 3 hours whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 10 kDa
Observed MW: 11 kDa
CXCL1 Recombinant Rabbit mAb (S-1856-35)
CXCL1 Recombinant Rabbit mAb (S-1856-35)
Price:
Regular price
$100 USD
Regular price
Sale price
$100 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CXCL1 |
| Synonyms | Growth-regulated alpha protein; C-X-C motif chemokine 1; Platelet-derived growth factor-inducible protein KC; Secretory protein N51; Gro; Gro1; Mgsa; Scyb1; Cxcl1 |
| Immunogen | Recombinant Protein |
| Location | Secreted |
| Accession | P12850 |
| Clone Number | S-1856-35 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM |
| Reactivity | Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Ms |
| ICC | 1:500 | Ms |
| ICFCM | 1:500 | Ms |
Background
CXCL1, also known as Keratinocyte Chemoattractant (KC), is a chemokine protein that plays a crucial role in immune responses. It is primarily produced by various cell types, including macrophages and endothelial cells, in response to inflammatory stimuli. CXCL1/KC functions by attracting neutrophils to sites of infection or tissue damage, thereby facilitating the immune system's ability to combat pathogens and initiate the healing process. This protein is also involved in the regulation of cell proliferation and angiogenesis, making it an important factor in both acute and chronic inflammatory conditions. Its expression and activity are tightly regulated to ensure appropriate immune responses and tissue homeostasis.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of 4% PFA fixed and Fixation/Permeabilization solution permeabilized RAW264.7, Stimulated 6 hours with 100ng/ml LPS and the last 3 hours with 300ng/ml BFA (Red) or untreated (Left panel) labelling CXCL1 antibody at 1/500 dilution (0.1 μg). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Immunocytochemistry
ICC analysis of Raw264.7 cells treated with LPS (100 ng/ml, 6h) and Brefeldin A (300 ng/ml, 3 h) (top panel) and untreated Raw264.7 cells (below panel). Anti- CXCL1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
