WB result of Choline Acetyltransferase/ChAT Recombinant Rabbit mAb
Primary antibody: Choline Acetyltransferase/ChAT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse kidney lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Negative control: mouse kidney lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 70 kDa
Choline Acetyltransferase/ChAT Recombinant Rabbit mAb (S-2561-17)
Choline Acetyltransferase/ChAT Recombinant Rabbit mAb (S-2561-17)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Choline Acetyltransferase/ChAT |
| Synonyms | Choline O-acetyltransferase; CHOACTase; Choline acetylase |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Synapse |
| Accession | P28329 |
| Clone Number | S-2561-17 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Human brain, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
Background
Choline acetyltransferase (ChAT) is a 69-kDa cytosolic transferase enzyme encoded by the CHAT gene that catalyzes the single-step acetylation of choline with acetyl-CoA to synthesize the neurotransmitter acetylcholine (ACh) in the presynaptic terminals of cholinergic neurons throughout the central and peripheral nervous systems; it is synthesized in the soma, trafficked to nerve endings via axoplasmic flow, exists in soluble and membrane-bound pools, displays alternative splicing that can generate larger nuclear variants, and is considered the most specific phenotypic marker for cholinergic neurons whose functional integrity underlies memory, cognition, arousal, and motor control, while its loss or dysfunction is closely linked to the cholinergic deficits seen in Alzheimer’s disease, amyotrophic lateral sclerosis, schizophrenia, and other neurodegenerative or neuropsychiatric disorders .
Picture
Picture
Western Blot
WB result of Choline Acetyltransferase/ChAT Recombinant Rabbit mAb
Primary antibody: Choline Acetyltransferase/ChAT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 82 kDa
Observed MW: 70 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-Choline Acetyltransferase/ChAT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Choline Acetyltransferase/ChAT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Choline Acetyltransferase/ChAT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
