WB result of Caspase-1 Recombinant Rabbit mAb
Primary antibody: Caspase-1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: EL4 whole cell lysate 20 µg
Lane 3: J774A.1 whole cell lysate 20 µg
Lane 4: A20 whole cell lysate 20 µg
Negative control: NIH/3T3 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 46 kDa
Caspase-1 Recombinant Rabbit mAb (S-2677-22)
Caspase-1 Recombinant Rabbit mAb (S-2677-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Caspase-1 |
| Synonyms | CASP-1; Interleukin-1 beta convertase (IL-1BC); Interleukin-1 beta-converting enzyme (ICE; IL-1 beta-converting enzyme); p45; Il1bc; Casp1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P29452 |
| Clone Number | S-2677-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Ms |
| Positive Sample | EL4, J774A.1, A20 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Ms |
| IHC-P | 1:250 | Ms |
Background
Caspase-1, the canonical member of the inflammatory caspase subfamily, is synthesized as a 45 kDa zymogen that assembles into the ~700 kDa inflammasome platform via homotypic CARD–CARD interactions with ASC and NLRP proteins; proximity-induced dimerization and trans-autoproteolysis generate the active p20/p10 tetramer whose p10 subunit forms a heterodimeric active site that cleaves the tetrapeptide motifs WEHD and YVAD, liberating the pro-inflammatory cytokines IL-1β and IL-18, triggering pyroptosis through gasdermin-D pore formation, and thereby orchestrating innate immune defense, metabolic regulation, and, when dysregulated, autoinflammatory diseases such as CAPS, atherosclerosis, and Alzheimer’s pathology.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded mouse colon. Anti- Caspase-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat lung. Anti- Caspase-1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
