WB result of Calnexin Rabbit pAb
Primary antibody: Calnexin Rabbit pAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: U-2 OS whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: THP-1 whole cell lysate 20 µg
Lane 5: SH-SY5Y whole cell lysate 20 µg
Lane 6: HepG2 whole cell lysate 20 µg
Lane 7: LNCaP whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 68 kDa
Observed MW: 100 kDa
Calnexin Rabbit Polyclonal Antibody
Calnexin Rabbit Polyclonal Antibody
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Calnexin |
| Synonyms | IP90; Major histocompatibility complex class I antigen-binding protein p88; p90; CANX |
| Immunogen | Synthetic Peptide |
| Location | Endoplasmic reticulum |
| Accession | P27824 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, U-2 OS, MCF7, THP-1, SH-SY5Y, HepG2, LNCaP |
| Purification | Immunogen Affinity |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
Calnexin is an integral endoplasmic reticulum (ER) type I transmembrane lectin chaperone that transiently binds newly synthesized monoglucosylated N-glycoproteins through its luminal carbohydrate-binding domain, coordinates sequential folding events with the oxidoreductase ERp57 to form disulfide bonds, retains incompletely folded or misassembled polypeptides in the ER via a cytosolic phosphorylation-regulated retrieval motif that interacts with the Sec61 translocon, and thereby serves as a central quality-control checkpoint that prevents aggregation and premature trafficking, while also participating in ER calcium homeostasis and cellular stress responses such as the unfolded protein response (UPR).
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-Calnexin antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti- Calnexin antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
