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Biotin Recombinant Rabbit mAb (S-3083)

Biotin Recombinant Rabbit mAb (S-3083)

Catalog Number: S0B6168 Application: WB Reactivity: SpeciesIndependent Conjugation: Unconjugated Brand: Starter
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Regular price $100 USD
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Product Details

Product Specification


Host Rabbit
Antigen Biotin
Clone Number S-3083
Antibody Type Recombinant mAb
Isotype IgG
Application WB
Reactivity Species Independent
Purification Protein A
Concentration 0.5 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

Stability & Storage

12 months from date of receipt / reconstitution, -20 °C as supplied

Background

Biotin, also known as vitamin B7 or vitamin H, is a water-soluble B-complex vitamin that serves as a crucial coenzyme for carboxylases, facilitating key biochemical processes such as gluconeogenesis, fatty acid synthesis, and amino acid metabolism. Since the human body cannot synthesize biotin on its own, it must be obtained through dietary sources (e.g., egg yolks, nuts, and animal liver) or in small amounts via gut microbiota. Its molecular structure consists of a ureido ring fused with a tetrahydrothiophene ring, and the valeric acid side chain enables binding to enzyme proteins for biological activity. Although widely believed to strengthen hair and nails due to its affinity for keratin, scientific evidence primarily supports biotin supplementation for addressing deficiencies or metabolic disorders, which may manifest as dermatitis, hair loss, or neurological abnormalities. The recommended daily intake for adults is approximately 30 μg, with higher doses needed for pregnant women or specific populations. While biotin toxicity is rare, excessive intake can interfere with laboratory tests (e.g., thyroid hormones, cardiac biomarkers).

Picture

Western Blot

Western Blot analysis of extracts from HeLa, NIH/3T3 and C6 cells using anti- tubulin, biotin linked Antibody followed by Anti Biotin rabbit mAb and anti- rabbit IgG, HRP linked Antibody.

Western Blot analysis of “Biotinylated Guanylyl Cyclase C/GUCY2C Fc&Avi Tag Protein, Human” using anti Biotin rabbit mAb followed by anti- rabbit IgG, HRP linked Antibody.

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