WB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HEK-293 whole cell lysate 20 µg
Lane 2: HepG2 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HT-1080 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrate
AGTR1 Recombinant Rabbit mAb (S-2447-22)
AGTR1 Recombinant Rabbit mAb (S-2447-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | AGTR1 |
| Synonyms | Type-1 angiotensin II receptor; AT1AR; AT1BR; Angiotensin II type-1 receptor (AT1 receptor); AGTR1A; AGTR1B; AT2R1; AT2R1B |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P30556 |
| Clone Number | S-2447-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HEK-293, HepG2, HeLa, HT-1080, C2C12, PC-12, COS-7 |
| Predicted Reactivity | Bv, Cz, Sh, GP, Dg, Rb |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:50-1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
AGTR1, the angiotensin II type 1 receptor, is a seven-transmembrane G-protein-coupled receptor that serves as the principal transducer of angiotensin II signaling in the renin–angiotensin–aldosterone system, mediating potent vasoconstriction, aldosterone and vasopressin release, renal sodium and water reabsorption, vascular smooth-muscle and cardiac hypertrophy, oxidative stress generation and sympathetic activation, thereby critically regulating systemic blood pressure and cardiovascular homeostasis; the receptor is widely expressed in kidney, heart, vasculature, adrenal gland and lung, can be activated not only by ligand binding but also by mechanical stretch, is transcriptionally up-regulated by inflammatory cytokines, undergoes endocytosis and heterodimerization that fine-tune its signaling output, and its chronic overactivity drives hypertension, cardiac and renal fibrosis, atherosclerosis and diabetic complications, while genetic polymorphisms that increase its expression or alter its regulation are linked to salt-sensitive hypertension and heightened cardiovascular risk .
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Picture
Western Blot
WB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C2C12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrate
WB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrate
WB result of AGTR1 Recombinant Rabbit mAb
Primary antibody: AGTR1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 41 kDa
Observed MW: 45 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-AGTR1 antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cardiac muscle. Anti-AGTR1 antibody was used at 1/50 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HEK-293 cells. Anti-AGTR1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
