Standard curve
Example of Monkey IgM standard curve in Assay Diluent #1.
Monkey IgM OneStep ELISA Kit
Monkey IgM OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | IgM |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Mk |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 2.8%； Inter-assay: 3.8% |
| Sample type | Cell culture supernatant, Serum, Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.246 ng/ml |
| Range | 0.469 ng/mL – 30 ng/mL |
| Recovery | Cell culture supernatant: 91% Serum: 90% Plasma: 90% |
| Assay time | 60 minutes |
| Species reactivity | Monkey |
Background
Monkey IgM, the largest antibody isotype with a pentameric structure (approximately 900 kDa) and 10 antigen-binding sites, serves as the essential first line of defense in the primary immune response, mediating initial pathogen neutralization and complement activation while being largely confined to the serum compartment due to its size. In monkeys (e.g., Macaca mulatta), IgM-positive B-cells develop progressively from low levels in infants, reaching near-adult levels around 5 months, with highly variable expression (26.3–94.9%) observed in adults—a critical factor for baseline screening in research models like transplantation studies. Careful sample handling (centrifugation, storage at -70°C) and awareness of assay cross-reactivity are important technical considerations. Commercially available kits and reagents enable precise quantification and analysis across clinical diagnostics and biomedical research, underscoring IgM's critical role in primate immunity.
Picture
Picture
ELISA
Linearity
The concentrations of IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: monkey serum was 3x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 701.44 ug/mL in monkey serum.
#2 sample is undiluted samples are as follows: monkey serum was 3x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 951.43 ug/mL in monkey serum.
#3 sample is undiluted samples are as follows: monkey serum was 3x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 756.01 ug/mL in monkey serum.
#4 sample is undiluted samples are as follows: monkey serum was 3x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 741.93 ug/mL in monkey serum.
#5 sample is undiluted samples are as follows: monkey serum was 3.2x10^4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 916.51 ug/mL in monkey serum.
To assess the linearity of the assay, two samples were spiked with high concentrations of Monkey IgM in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, The samples was spiked with high concentrations of Monkey IgM in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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