IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Uroplakin III antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Uroplakin III Recombinant Rabbit mAb (SDT-R177)
Uroplakin III Recombinant Rabbit mAb (SDT-R177)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Uroplakin III |
| Synonyms | Uroplakin-3a, UP3a, UPIII |
| Location | Endoplasmic reticulum membrane |
| Accession | O75631 |
| Clone Number | SDT-R177 |
| Antibody Type | Recombinant mAb |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC | 1:1000 | null |
Background
Uroplakin III is found in the inner membrane of the urinary bladder and contributes to the strength of that membrane and to the membrane's ability to stretch when the bladder is full. Uroplakin III can be expressed in primary and invasive bladder epithelial carcinomas, but is not expressed in many non-urothelial tumors, thus contributing to the research of bladder epithelial carcinoma. The Uroplakin III gene is deleted in normal urothelium and in bladder cancer after malignant metastasis, which can manifest as urothelial tumors with malignant tendencies and can be used as a reference for cell differentiation of urothelial cells. Uroplakin III-positive bladder epithelial cancer patients have a high relative survival rate.
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Immunohistochemistry
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-Uroplakin III antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human hepatocellular carcinoma. Anti-Uroplakin III antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-Uroplakin III antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-Uroplakin III antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human liver. Anti-Uroplakin III antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
