WB result of TCF-4 Recombinant Rabbit mAb
Primary antibody: TCF-4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Daudi whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 55-65 kDa
This blot was developed with high sensitivity substrate
TCF-4 Recombinant Rabbit mAb, PBS Only (SDT-2549-4)
TCF-4 Recombinant Rabbit mAb, PBS Only (SDT-2549-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TCF-4 |
| Synonyms | Transcription factor 4; Class B basic helix-loop-helix protein 19 (bHLHb19); Immunoglobulin transcription factor 2 (ITF-2); SL3-3 enhancer factor 2 (SEF-2); BHLHB19; ITF2; SEF2; TCF4 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P15884 |
| Clone Number | SDT-2549-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Daudi, SH-SY5Y, Neruo-21, A20 |
| Purification | Protein A |
| Concentration | 1 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS |
| Stability & Storage | 12 months from date of receipt / reconstitution, 4 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:250 | Hu, Ms, Rt |
Background
TCF-4 (Transcription Factor 4), a 667-amino-acid basic helix–loop–helix protein encoded by the TCF4 gene on chromosome 18q21.2, is a master regulator of neurodevelopment, immune tolerance and epithelial homeostasis that binds the canonical E-box motif 5′-CACCTG-3′ to activate or repress transcription in partnership with β-catenin in the Wnt pathway, interacts with HDACs and CBP/p300 to modulate chromatin state, and whose dosage-sensitive expression is critically linked to Pitt–Hopkins syndrome and schizophrenia while its somatic amplifications and activating mutations drive colorectal, pancreatic and breast tumorigenesis by enforcing a stem-like transcriptional program.
Picture
Picture
Western Blot
WB result of TCF-4 Recombinant Rabbit mAb
Primary antibody: TCF-4 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: A20 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 71 kDa
Observed MW: 55-80 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon cancer. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse spleen. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat spleen. Anti- TCF-4 antibody was used at 1/250 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
