Tau Recombinant Mouse mAb (SDT-R145)
Tau Recombinant Mouse mAb (SDT-R145)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | Tau |
| Synonyms | Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau |
| Immunogen | N/A |
| Location | Cytoplasm, Secreted, Cell membrane |
| Accession | P10636 |
| Clone Number | SDT-R145 |
| Isotype | IgG1 |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IHC-P | 1:2000 | null |
Background
Tau is a microtubule-associated protein for which the physiological functions remain a topic of vigorous investigation. Additionally, tau is a central player in the pathogenesis of several diseases such as Alzheimer’s disease and several frontotemporal dementias. A critical variable to understanding tau in physiological and disease contexts is its normal localization within cells of the adult CNS.
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Western Blot
WB result of Tau mouse mAb Primary antibody: Tau mouse mAb at 1/1000 dilution Lane 1: mouse liver lysate 20 µg Lane 2: mouse brain lysate 20 µg Negative control: mouse liver lysate Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 50~65 kDa Observed MW: 50~65 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-Tau antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
