WB result of Smad4 Rabbit mAb
Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
Lane 1: SW480 whole cell lysate 20 µg
Lane 2: HT-29 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Negative control: SW480 whole cell lysate;
HT-29 whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 65 kDa
Exposure time: 180s
SMAD4 Recombinant Rabbit mAb (SDT-168-67)
SMAD4 Recombinant Rabbit mAb (SDT-168-67)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Smad4 |
| Synonyms | Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; hSMAD4 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q13485 |
| Clone Number | SDT-168-67 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IP | 1:50 | Hu, Ms, Rt |
Background
SMAD (mothers against decapentaplegic homologs) molecules are the core components in TGF-β signaling pathway. TGF-β binding to its receptor induces phosphorylation and activation of receptor-regulated SMADs (R-SMADs), SMAD2 and SMAD3, which subsequently associate with their partner SMAD4 and translocate from cytoplasm to nucleus. Formation of R-SMAD–SMAD4 complexes is essential in signaling of most TGF-β family members.
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Western Blot
WB result of Smad4 Rabbit mAb
Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 65 kDa
Exposure time: 180s
WB result of Smad4 Rabbit mAb
Primary antibody: Smad4 Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 70 kDa
Observed MW: 65 kDa
Exposure time: 180s
IP
SMAD4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating SMAD4 in 0.4 mg HeLa whole cell lysate.
Western blot was performed on the immunoprecipitate using SMAD4 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 20 µg (Input)
Lane 2: SMAD4 Rabbit mAb IP in HeLa whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HeLa whole cell lysate
Predicted MW: 70 kDa
Observed MW: 70 kDa
