| Host | Rabbit |
| Antigen | Chromogranin A |
| Synonyms | CgA, Pituitary secretory protein I, SP-I |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P10645 |
| Clone Number | SDT-074-57 |
| Antibody Type | Recombinant mAb |
| Application | WB, IHC-P, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv, Pg |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Tag | N/A |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| WB | 1:500 | |
| IHC-P | 1:1000-1:2000 | |
| IP | 1:25 |
Chromogranin A (CgA) is a prohormone and granulogenic factor in endocrine and neuroendocrine tissues, as well as in neurons, and has a regulated secretory pathway [PMID: 28228748]. Chromogranin A is known as an important marker of neuroendocrine tumors [PMID: 24325234]. The intracellular functions of CgA include the initiation and regulation of dense-core granule biogenesis and sequestration of hormones in neuroendocrine cells. This protein is co-stored and co-released with secreted hormones. The extracellular functions of CgA include the generation of bioactive peptides, such as pancreastatin (PST), vasostatin, WE14, catestatin (CST), and serpinin [PMID: 28228748].
WB result of Chromogranin A Rabbit mAb
Primary antibody: Chromogranin A Rabbit mAb at 1/500 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: SH-SY5Y whole cell lysate 20 µg
Lane 3: TT whole cell lysate 20 µg
Lane 4: Neuro-2a whole cell lysate 20 µg
Negative control: Jurkat whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50kDa
Observed MW: 20, 75kDa
WB result of Chromogranin A Rabbit mAb
Primary antibody: Chromogranin A Rabbit mAb at 1/500 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 50kDa
Observed MW: 80, 90kDa
Chromogranin A Rabbit mAb at 1/25 dilution (1 µg) immunoprecipitating Chromogranin A in 0.4 mg PC-12 whole cell lysate.
Western blot was performed on the immunoprecipitate using Chromogranin A Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: PC-12 whole cell lysate 40 µg (Input)
Lane 2: Chromogranin A Rabbit mAb IP in PC-12 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in PC-12 whole cell lysate
Predicted MW: 50 kDa
Observed MW: 80,90 kDa
（This blot was developed with high sensitivity substrate）
IHC shows positive staining in paraffin-embedded human pancreas. Anti-Chromogranin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Chromogranin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human medullary thyroid carcinoma. Anti-Chromogranin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pheochromocytoma. Anti-Chromogranin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human small cell lung carcinoma. Anti-Chromogranin A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse pancreas. Anti-Chromogranin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-Chromogranin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat pancreas. Anti-Chromogranin A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
