1 1

LY9/CD229 Recombinant Rabbit mAb, PBS Only (SDT-2393-47)

LY9/CD229 Recombinant Rabbit mAb, PBS Only (SDT-2393-47)

Catalog Number: S0B2430P Application: WB, IHC-P Reactivity: Human Conjugation: Unconjugated Brand: Starter
Share:
Price:
$131.00 SGD
$131.00 SGD
Size:
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details

Product Details

Product Specification


Host Rabbit
Antigen LY9/CD229
Synonyms T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3 (SLAMF3); Signaling lymphocytic activation molecule 3
Immunogen Recombinant Protein
Location Cell membrane
Accession Q9HBG7
Clone Number SDT-2393-47
Antibody Type Recombinant mAb
Isotype IgG
Application WB, IHC-P
Reactivity Hu
Positive Sample Daudi, Jurkat
Purification Protein A
Concentration 1 mg/ml
Conjugation Unconjugated
Physical Appearance Liquid
Storage Buffer

PBS
Stability & Storage

12 months from date of receipt / reconstitution, 4°C as supplied

Dilution


application dilution species
WB 1:1000 Hu
IHC-P 1:1000 Hu

Background

CD229, also known as LY9 or SLAMF3, is a 120 kDa type I transmembrane glycoprotein of the SLAM subgroup of the CD2 family, expressed on T, B, NK, dendritic and stem cells, that functions as a self-ligand through homophilic binding of its N-terminal IgV-like domain to regulate immune responses. Upon engagement, phosphorylation of two ITSM motifs in its long cytoplasmic tail recruits the adaptor proteins SAP or EAT-2 (or the phosphatases SHP-2/SHIP1), leading to signals that can either enhance or inhibit leukocyte activation, differentiation, cytokine production and cytotoxicity, depending on cellular context, and thereby controls processes such as T-cell adhesion and immunological synapse formation, Th17 polarization, maintenance of peripheral tolerance and prevention of autoimmunity, while its overexpression on malignant plasma and memory B cells has made CD229 a prognostic biomarker and CAR-T target in multiple myeloma and other B-cell malignancies.

Picture

Western Blot

WB result of LY9/CD229 Recombinant Rabbit mAb
Primary antibody: LY9/CD229 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U937 whole cell lysate 20 µg
Lane 2: Daudi whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Negative control: U937 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 72 kDa
Observed MW: 60-130 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human myeloma. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung cancer. Anti-LY9/CD229 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.