Hemoglobin Recombinant Rabbit mAb (SDT-193-71)

Catalog Number: S0B0057 Application: WB,IHC-P,IP Reactivity: Human Conjugation: Unconjugated Brand: Starter

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$131.00 SGD

Size: 25μl 100μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Hemoglobin
Synonyms	Hb
Immunogen	Native protein
Location	Blood microparticle, Cytosol
Accession	P69905、P68871
Clone Number	SDT-193-71
Antibody Type	Rabbit mAb
Application	WB, IHC-P, IP
Reactivity	Hu
Purification	Protein A
Concentration	0.25 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:500	null
IHC-P	1:5000	null
IP	1:25	null

Background

The primary function of Hemoglobin (Hb) is to transport oxygen (O2) from the lung to tissues, binding and releasing O2 in a cooperative manner [PMID: 32189307]. Hemoglobin is a tetramer, consisting of two α-subunits (α1 and α2) and two βsubunits (β1 and β2) that are structurally similar and about the same size. The two αβ dimers (named α1β1 and α2β2) are arranged around a 2-fold axis of symmetry resulting in a large central water cavity in the T or unliganded or deoxygenated structure and a narrower cavity in the R or liganded or oxygenated structure.

Picture

Western Blot

WB result of Hemoglobin Rabbit mAb Primary antibody: Hemoglobin Rabbit mAb at 1/500 dilution Lane 1: 293T whole cell lysate 20 µg Lane 2: TF-1 whole cell lysate 20 µg Negative control: 293T whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 13 kDa Observed MW: 13 kDa

IP

Hemoglobin Rabbit mAb at 1/25 dilution (1µg) immunoprecipitating Hemoglobin in 0.4mg TF-1 whole cell lysate.
Western blot was performed on the immunoprecipitate using Hemoglobin Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1 : TF-1 whole cell lysate 20µg(input)
Lane 2 : Hemoglobin Rabbit mAb IP in TF-1 whole cell lysate
Lane 3 : Rabbit monoclonal IgG IP in TF-1 whole cell lysate
Predicted MW: 13 kDa
Observed MW: 13 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human tonsil. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human placenta. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human prostate. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-Hemoglobin antibody was used at 1/5000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.