IHC shows positive staining in paraffin-embedded human cerebellum. Anti-GFAP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
GFAP Recombinant Mouse mAb (SDT-3032)
GFAP Recombinant Mouse mAb (SDT-3032)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | GFAP |
| Synonyms | Glial fibrillary acidic protein |
| Location | Cytoplasm |
| Accession | P14136 |
| Clone Number | SDT-3032 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG1,k |
| Application | IHC-P |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000 | Hu |
Background
Glial fibrillary acidic protein (GFAP) is a type of intermediate filament protein that is primarily expressed in astrocytes, which are a kind of glial cells in the central nervous system. It plays a crucial role in maintaining the structural integrity of astrocytes and providing support to neurons. GFAP helps to anchor astrocytes to the extracellular matrix and other cells, contributing to the stability of the brain tissue. Additionally, it is involved in various cellular processes such as cell signaling, migration, and proliferation of astrocytes. In clinical practice, elevated levels of GFAP in cerebrospinal fluid or blood can be an indicator of certain neurological disorders like traumatic brain injury, brain tumors, and neurodegenerative diseases, making it a potentially valuable biomarker for diagnosis and monitoring of these conditions.
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Immunohistochemistry
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-GFAP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-GFAP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human breast cancer. Anti-GFAP antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
