WB result of GATA3 Recombinant Mouse mAb
Primary antibody: GATA3 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: T-47D whole cell lysate 20 µg
Secondary antibody: Goat Anti-mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
GATA3 Recombinant Mouse mAb (SDT-3180)
GATA3 Recombinant Mouse mAb (SDT-3180)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | GATA3 |
| Synonyms | Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3 |
| Location | Nucleus |
| Accession | P23771 |
| Clone Number | SDT-3180 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | T-47D, EL4 |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:4000 | Hu, Ms, Rt |
Background
GATA3 (GATA-binding protein 3) is a zinc-finger transcription factor encoded by the GATA3 gene on chromosome 10p15 that orchestrates embryogenesis, cell fate, and differentiation across epithelial, endothelial, hematopoietic, and neural lineages by binding the consensus WGATAR DNA motif, and its indispensable roles in mammary luminal specification, T-helper-2 immune responses, parathyroid and inner-ear development are underscored by the severe multi-organ defects seen in Gata3-haploinsufficient HDR syndrome, while in cancer its context-dependent function manifests as an estrogen-receptor-coactivating oncogene in luminal breast tumors and a lineage-survival oncogene in urothelial carcinomas, yet also as a tumor suppressor in some contexts, making immunohistochemical GATA3 positivity a widely used diagnostic biomarker for carcinomas of breast and urothelial origin and a potential therapeutic target whose modulation is being explored with small-molecule inhibitors and epigenetic strategies.
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Western Blot
WB result of GATA3 Recombinant Mouse mAb
Primary antibody: GATA3 Recombinant Mouse mAb at 1/1000 dilution
Lane 1: EL4 whole cell lysate 20 µg
Secondary antibody: Goat Anti- mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 48 kDa
Observed MW: 48 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human kidney. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human bladder cancer. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon cancer. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-GATA3 antibody was used at 1/4000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
