IF shows positive staining in paraffin-embedded human breast cancer. Anti- Cytokeratin 7 (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution (magenta) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Cytokeratin 7 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-R028)
Cytokeratin 7 Recombinant Rabbit mAb (Alexa Fluor® 700 Conjugate) (SDT-R028)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cytokeratin 7 |
| Synonyms | Cytokeratin-7 (CK-7), Keratin-7 (K7), Sarcolectin, Type-II keratin Kb7, KRT7 |
| Location | Cell membrane, Intracellular |
| Accession | P08729 |
| Clone Number | SDT-R028 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2.5 mg/ml |
| Conjugation | Alexa Fluor® 700 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:100 |
Background
Cytokeratin 7 is the intermediate silk protein mainly expressed in epithelial cells. The heterodine of keratin consists of a polymerization filament by an acid -keratin (or type I keratin, keratin 9 to 23) and alkaline keratin (or type II keratin, keratin 1 to 8). Expression similar to but more limited than keratins 8 and 18 in simple, pseudostratified and ductal epithelium, mesothelium and urothelium. It's generally expressed (with some variation) in adenocarcinoma of lung, breast, thyroid, endometrium, cervix, ovary, salivary gland, upper GI tract, urothelial carcinoma, papillary renal cell carcinoma and Paget disease. It's generally negative (with some variation) in colorectal carcinoma, Merkel cell carcinoma, hepatocellular carcinoma, prostatic adenocarcinoma, adrenocortical tumors and squamous cell carcinoma.
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Immunofluorescence
Negative control: IF shows negative staining in paraffin-embedded human gastrointestinal stromal tumor. Anti- Cytokeratin 7 (Alexa Fluor® 700 Conjugate) antibody was used at 1/100 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
