IF shows positive staining in paraffin-embedded human breast cancer. Anti-Cytokeratin 19 fragment (CY211) (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Cytokeratin 19 fragment (CY211) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-197-28)
Cytokeratin 19 fragment (CY211) Recombinant Rabbit mAb (Alexa Fluor® 488 Conjugate) (SDT-197-28)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Cytokeratin 19 fragment (CY211) |
| Synonyms | CYFRA 21-1 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Membrane |
| Accession | P08727 |
| Clone Number | SDT-197-28 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 488 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:200 | null |
Background
Cytokeratin 19 fragment (CYFRA 21-1) is a fragment of cytokeratin 19 that is typically associated with epithelial cell cancers, including NSCLC, and is typically associated with the SQLC type. Since cytokeratins are structural proteins of keratin-containing intermediate filaments found in the epithelial cells, their degradation produces soluble fragments which are measurable in the blood of lung cancer patients as a tumor marker. Other examples of cytokeratin fragments include tissue polypeptide antigen and tissue polypeptide-specific antigen. CYFRA 21-1 is known to be correlated with disease response and the prognosis of lung cancer but cannot be used to differentiate cancer patients from patients with respiratory diseases.
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Immunofluorescence
IF shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Cytokeratin 19 fragment (CY211) (Alexa Fluor® 488 Conjugate) antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
