CD4 Recombinant Rabbit mAb (SDT-R163)

Catalog Number: S0B2179 Application: WB,IHC-P,FCM,IP,IF Reactivity: Human Conjugation: Unconjugated Brand: Starter

Price:

$100 USD

Size: 25μl 100 μl 500μl

Quantity:

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Product Details

Product Specification

Host	Rabbit
Antigen	CD4
Synonyms	T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4
Immunogen	N/A
Location	Cell membrane
Accession	P01730
Clone Number	SDT-R163
Antibody Type	Recombinant mAb
Application	WB, IHC-P, FCM, IP, IF
Reactivity	Hu
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000	null
IHC-P	1:500	null
FCM	1:50	null
IP	1:50	null
IF	1:200	null

Background

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells. It is a type of white blood cell that helps fight infection by triggering your immune system to destroy viruses, bacteria, and other germs that may make you sick. CD4 is also a receptor for the HIV virus, and when the virus infects cells with CD4 surface proteins, it depletes the number of T cells, B cells, natural killer cells, and monocytes in the patient's blood.

Picture

Western Blot

WB result of CD4 Rabbit mAb Primary antibody: CD4 Rabbit mAb at 1/1000 dilution Lane 1: Ramos whole cell lysate 20 µg Lane 2: THP-1 whole cell lysate 20 µg Lane 3: Jurkat whole cell lysate 20 µg Lane 4: Molt-4 whole cell lysate 20 µg Negative control: Ramos whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 51kDa Observed MW: 55kDa

FC

Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labelling CD4 antibody at 1/50 (1 μg) dilution (Right) compared with a Rabbit monoclonal IgG isotype control (Left). Goat Anti – Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody, cells were stained with CD3 - Alexa Fluor® 647 simultaneously. Events were gated on viable lymphocytes.

IP

CD4 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD4 in 0.4 mg THP-1 whole cell lysate.
Western blot was performed on the immunoprecipitate using CD4 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: THP-1 whole cell lysate 20 µg (Input)
Lane 2: CD4 Rabbit mAb IP in THP-1 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in THP-1 whole cell lysate
Predicted MW: 51 kDa
Observed MW: 55 kDa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human thymus. Anti-CD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen. Anti-CD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human liver. Anti-CD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human cerebral cortex. Anti-CD4 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunofluorescence

IF shows positive staining in paraffin-embedded human tonsil. Anti-CD4 antibody was used at 1/200 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.