IF shows positive staining in paraffin-embedded human tonsil. Anti-CD19 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
CD19 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-164-41)
CD19 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-164-41)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD19 |
| Synonyms | B-lymphocyte antigen CD19; LE-CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P15391 |
| Clone Number | SDT-164-41 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IF |
| Reactivity | Hu |
| Positive Sample | Human tonsil, Human diffuse large B-cell lymphoma |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Alexa Fluor® 555 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| IF | 1:200 | Hu |
Background
The CD19 antigen plays an important role in clinical oncology. In normal cells, it is the most ubiquitously expressed protein in the B lymphocyte lineage. CD19 expression is induced at the point of B lineage commitment during the differentiation of the hematopoietic stem cell, and its expression continues through preB and mature B cell differentiation until it is finally down-regulated during terminal differentiation into plasma cells. CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation, and therefore CD19 is useful in diagnosis of leukemias and lymphomas using monoclonal antibodies (mAbs) and flow cytometry.
Picture
Picture
Immunofluorescence
IF shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma. Anti-CD19 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
