WB result of CD133 Recombinant Rabbit mAb
Primary antibody: CD133 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Lane 3: HT-29 whole cell lysate 20 µg
Lane 4: COLO 205 whole cell lysate 20 µg
Lane 5: SW620 whole cell lysate 20 µg
Lane 6: HCT116 whole cell lysate 20 µg
Negative control: 293T whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 97 kDa
Observed MW: 110~190 kDa
CD133 Recombinant Rabbit mAb(SDT-1295-22)
CD133 Recombinant Rabbit mAb(SDT-1295-22)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | CD133 |
| Synonyms | Prominin-1; Antigen AC133; Prominin-like protein 1; PROM1; PROML1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | O43490 |
| Clone Number | SDT-1295-22 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| IHC-P | 1:1000 |
Background
CD133, also known as Prominin-1, is a pentaspan membrane glycoprotein expressed on the surface of various stem cells and cancer stem cells (CSCs). It plays a crucial role in tumorigenesis, metastasis, recurrence, and drug resistance, and is involved in the regulation of cellular energy metabolism, affecting glucose uptake, autophagy, and ATP synthesis. Moreover, the function of CD133 is not limited to cancer cells; it is also expressed in differentiated cells and stem/progenitor cells in normal tissues and is involved in multiple cellular processes, coordinating signaling pathways that are often deregulated in cancer, such as the PI3K/Akt signaling and Wnt/β-catenin pathways.
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Western Blot
IP
CD133 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating CD133 in 0.4 mg HT-29 whole cell lysate.
Western blot was performed on the immunoprecipitate using CD133 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: HT-29 whole cell lysate 5 µg (Input)
Lane 2: CD133 Rabbit mAb IP in HT-29 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HT-29 whole cell lysate
Predicted MW: 97 kDa
Observed MW: 110~190 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-CD133 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreas. Anti-CD133 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-CD133 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-CD133 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CD133 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
