IF shows positive staining in paraffin-embedded human ovarian cancer. Anti- CA125 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Product Details
Product Details
Product Specification
Host | Rabbit |
Antigen | CA125 |
Synonyms | Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, MUC16, Cancer antigen 125 |
Immunogen | Synthetic Peptide |
Location | Secreted, Cell membrane, Extracellular space |
Accession | Q8WXI7 |
Clone Number | SDT-555-41 |
Antibody Type | Recombinant mAb |
Isotype | IgG |
Application | IF |
Reactivity | Hu |
Purification | Protein A |
Concentration | 2 mg/ml |
Conjugation | Alexa Fluor® 555 |
Physical Appearance | Liquid |
Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
application | dilution | species |
IF | 1:200 | null |
Background
Mucin-16 (MUC-16), also known as ovarian cancer associated tumor marker CA125, is a protein encoded by the MUC16 gene. MUC-16 is a member of the glycoprotein family of mucins. MUC-16 has been applied as a tumor marker or biomarker, which may be elevated in the blood of certain types of cancer patients, especially those with cancer or other benign diseases. Detecting CA-125 blood levels is considered useful for treating ovarian cancer. Although this test can provide useful information for women who are known to have cancer, due to the uncertain correlation between CA-125 levels and cancer, the CA-125 test has not been found to be useful as a screening method. In addition to ovarian cancer, CA-125 also increases in patients with endometrial cancer, fallopian tube cancer, lung cancer, breast cancer, and gastrointestinal cancer. Pregnant women may also experience this condition in their bodies. Due to the fact that serum levels can be increased under various circumstances, CA-125 is not used for cancer detection, but it is typically used to monitor ovarian cancer patients' response to chemotherapy, recurrence, and disease progression.
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Immunofluorescence
Negative control: IF shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti- CA125 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
