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CA125 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-555-41)

CA125 Recombinant Rabbit mAb (Alexa Fluor® 555 Conjugate) (SDT-555-41)

Catalog Number: S0B1574 Application: IF Reactivity: Human Conjugation: Alexa Fluor 555 Brand: Starter
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Regular price $180 USD
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Product Details

Product Specification


Host Rabbit
Antigen CA125
Synonyms Mucin-16, MUC-16, Ovarian cancer-related tumor marker CA125, Ovarian carcinoma antigen CA125, MUC16, Cancer antigen 125
Immunogen Synthetic Peptide
Location Secreted, Cell membrane, Extracellular space
Accession Q8WXI7
Clone Number SDT-555-41
Antibody Type Recombinant mAb
Isotype IgG
Application IF
Reactivity Hu
Purification Protein A
Concentration 2 mg/ml
Conjugation Alexa Fluor® 555
Physical Appearance Liquid
Storage Buffer PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300
Stability & Storage 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.

Dilution


application dilution species
IF 1:200 null

Background

Mucin-16 (MUC-16), also known as ovarian cancer associated tumor marker CA125, is a protein encoded by the MUC16 gene. MUC-16 is a member of the glycoprotein family of mucins. MUC-16 has been applied as a tumor marker or biomarker, which may be elevated in the blood of certain types of cancer patients, especially those with cancer or other benign diseases. Detecting CA-125 blood levels is considered useful for treating ovarian cancer. Although this test can provide useful information for women who are known to have cancer, due to the uncertain correlation between CA-125 levels and cancer, the CA-125 test has not been found to be useful as a screening method. In addition to ovarian cancer, CA-125 also increases in patients with endometrial cancer, fallopian tube cancer, lung cancer, breast cancer, and gastrointestinal cancer. Pregnant women may also experience this condition in their bodies. Due to the fact that serum levels can be increased under various circumstances, CA-125 is not used for cancer detection, but it is typically used to monitor ovarian cancer patients' response to chemotherapy, recurrence, and disease progression.

Picture

Immunofluorescence

IF shows positive staining in paraffin-embedded human ovarian cancer. Anti- CA125 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution (Red) and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

Negative control: IF shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti- CA125 (Alexa Fluor® 555 Conjugate) antibody was used at 1/200 dilution and incubated overnight at 4°C. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.

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