| Host | Rabbit |
| Antigen | Bcl-2 |
| Immunogen | Synthetic Peptide |
| Accession | P10415 |
| Clone Number | SDT-005-24 |
| Antibody Type | Rabbit mAb |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Purification | Protein A |
| Research Area | Apoptosis |
| Concentration | 1 mg/ml |
| Molecular Weight | 26kDa |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| IHC-P | 1:500-1:2000 | |
| WB | 1:5000 |
Bcl-2 (B-cell lymphoma 2) is the founding member of the Bcl-2 family of regulator proteins that regulate cell death (apoptosis), by either inhibiting (anti-apoptotic) or inducing (pro-apoptotic) apoptosis. It was the first apoptosis regulator identified in any organism. Bcl-2 is localized to the outer membrane of mitochondria, where it plays an important role in promoting cellular survival and inhibiting the actions of pro-apoptotic proteins. Damage to the Bcl-2 gene has been identified as a cause of a number of cancers, including melanoma, breast, prostate, chronic lymphocytic leukemia, and lung cancer, and a possible cause of schizophrenia and autoimmunity. It is also a cause of resistance to cancer treatments.
WB result of anti-Bcl-2 antibody
Primary antibody : Anti-Bcl2 antibody at 1/5000 dilution
Lane 1 : Daudi whole cell lysate 20 µg
Lane 2 : Jurkat whole cell lysate 20 µg
Lane 3 : THP-1 whole cell lysate 20 µg
Lane 4 : MCF7 whole cell lysate 20 µg
Lane 5 : Hela whole cell lysate 20 µg
Negative control: Daudi whole cell lysate
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 26 kDa
Observed MW: 26 kDa
econds
IHC shows positive staining in paraffin-embedded human tonsil.
Anti-Bcl2 antibody was used at 1/250 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Counterstained with hematoxylin.
Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
