Asialoglycoprotein Receptor 1 Recombinant Rabbit mAb (SDT-292-48)

Catalog Number: S0B0088 Application: WB,IHC-P,ICC,FCM Reactivity: Human,Mouse,Rat Conjugation: Unconjugated Brand: Starter

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$100 USD

Size: 25ul 100 μl

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Product Details

Product Specification

Host	Rabbit
Antigen	Asialoglycoprotein Receptor 1
Synonyms	ASGP-R 1, ASGPR 1, C-type lectin domain family 4 member H1, Hepatic lectin H1, HL-1, ASGR1, CLEC4H1
Immunogen	Recombinant Protein
Location	Secreted, Membrane
Accession	P07306
Clone Number	SDT-292-48
Antibody Type	Recombinant mAb
Application	WB, IHC-P, ICC, ICFCM
Reactivity	Hu, Ms, Rt
Purification	Protein A
Concentration	0.5 mg/ml
Physical Appearance	Liquid
Storage Buffer	PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300
Stability & Storage	12 months from date of receipt / reconstitution, -20 °C as supplied

Dilution

application	dilution	species
WB	1:1000-1:5000	null
IHC-P	1:2000	null
ICFCM	1:5000	null
ICC	1:500	null

Background

Asialoglycoprotein receptors (ASGP-R; exist as subtypes 1 and 2) are located on the surface of hepatic cell membranes and are involved in the binding and endocytosis of glycoproteins that have exposed carbohydrate or N-acetylgalactosamine residues. In addition, the decreased level of ASGP-R on the hepatic cells has been shown to correlate clinically with the degree of liver function retained due to the development of cirrhosis, cancer, or viral hepatitis [PMID: 20641215].

Picture

Western Blot

WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/5000 dilution Lane 1: HeLa whole cell lysate 20 µg Lane 2: HepG2 whole cell lysate 20 µg Negative control: HeLa whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40~50kDa

WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/5000 dilution Lane 1: mouse liver lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40kDa

WB result of Asialoglycoprotein Receptor 1 Rabbit mAb Primary antibody: Asialoglycoprotein Receptor 1 Rabbit mAb at 1/1000 dilution Lane 1: rat liver lysate 20 µg Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 33kDa Observed MW: 40kDa

FC

Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, left) / HepG2 (Human hepatocellular carcinoma epithelial cell, Right) cells labelling Asialoglycoprotein Receptor 1 antibody at 1/5000 dilution (0.01 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.Negative control: HeLa

Immunohistochemistry

IHC shows positive staining in paraffin-embedded human liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded human lung squamous cell carcinoma. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-Asialoglycoprotein Receptor 1 antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Immunocytochemistry

ICC shows positive staining in HepG2 cells. Anti-ASGR1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).

Negative control：ICC shows negative staining in HeLa cells. Anti-ASGR1 antibody was used at 1/500 dilution and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue).Counterstain with tubulin (Red).