| Host | Rabbit |
| Antigen | Antithrombin III |
| Synonyms | ATIII, Serpin C1 |
| Immunogen | Recombinant Protein |
| Location | Secreted, Extracellular space |
| Accession | P01008 |
| Clone Number | SDT-294-211 |
| Antibody Type | Recombinant mAb |
| Application | WB, IP |
| Reactivity | Hu |
| Cross Reactivity | Does not recognize thrombin, PIC complex, tPA-PAI-1 complex |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Tag | N/A |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 |
Antithrombin III (AT III) is primarily a potent anticoagulant with independent anti-inflammatory properties. AT III irreversibly inhibits serine proteases (e.g. activated factor X and thrombin) in a one-to-one ratio, with the generation of protease–AT III complexes. Heparin prevents AT III from interacting with the endothelial cell surface by binding to sites on the AT III molecule, competing for the AT III binding site, and reducing AT III’s ability to interact with its cellular receptor [PMID: 26862016].
WB result of Antithrombin III Rabbit mAb
Primary antibody: Antithrombin III Rabbit mAb at 1/1000 dilution
Lane 1: Human serum lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 52kDa
Observed MW: 55kDa
Antithrombin III Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating Antithrombin III in 0.4 mg human serum lysate.
Western blot was performed on the immunoprecipitate using Antithrombin III Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: human serum lysate 10 µg (Input)
Lane 2: Antithrombin III Rabbit mAb IP in human serum lysate
Lane 3: Rabbit monoclonal IgG IP in human serum lysate
Predicted MW: 52 kDa
Observed MW: 55 kDa
（This blot was developed with high sensitivity substrate）
