ELISA binding of Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61) rabbit with Pembrolizumab. While the antibody partly cross react with the human IgG4 control (data not shown). When it is used as capture antibody in Pembrolizumab Sandwich ELISA, Sandwich ELISA reult show no cross reactivity with Human IgG4 control and human total IgG.
Coating antigen: Pembrolizumab, 0.2 µg/ml.
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61) (S0B0842) dilutions start from 1000 ng/ml.
EC50= 20.74ng/ml.
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61)
Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61)
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$420 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Pembrolizumab |
| Synonyms | Keytruda |
| Clone Number | SDT-825-61 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | ELISA, PK study |
| Purification | Protein A |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | Lyophilized with PBS pH7.4, 0.03% Proclin 300 |
| Reconstitution | Reconstitute the lyophilized powder with deionized water to a final concentration of 0.5 mg/ml. |
| Stability & Storage | The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles. |
Dilution
| application | dilution | species |
| ELISA | 0.01-0.1 µg/ml |
Background
Pembrolizumab, sold under the brand name Keytruda, is a humanized antibody used in cancer immunotherapy that treats melanoma, lung cancer, head and neck cancer, Hodgkin lymphoma, stomach cancer, cervical cancer, and certain types of breast cancer. It is administered by slow intravenous injection. It is an IgG4 isotype antibody that blocks a protective mechanism of cancer cells, allowing the immune system to destroy them. It targets the programmed cell death protein 1 (PD-1) receptor of lymphocytes by blocking its interaction with PD-L1, resulting in the activation of T-cell-mediated immune responses against tumor cells.
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ELISA
Paired Recommendations
Standard curve of Pembrolizumab Sandwich ELISA. The Pembrolizumab Sandwich ELISA assay is developed by using Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-61) (S0B0842) and Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) (S0B0840) as the capture and detection antibodies, respectively.
In this ELISA assay, Anti-Pembrolizumab Recombinant Rabbit mAb (SDT-825-21) (S0B0840) was labeled with Biotin.
The sensitivity of detecting Pembrolizumab is up to 23 pg/ml.
