WB result of Invivo anti-mouse CD8α Rat mAb
Primary antibody: Invivo anti-mouse CD8α Rat mAb at 1/50 dilution
Lane 1: Mouse CD8, His tag (S0A1018) 1 µg
Lane 2: Mouse CD8, His tag (S0A1018) 0.5 µg
Secondary antibody: Goat Anti-rat IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted MW: 35 kDa
Observed MW: 25~35 kDa
Invivo anti-mouse CD8α Recombinant mAb
Invivo anti-mouse CD8α Recombinant mAb
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$196.00 SGD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Synonyms | T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, Lyt-2, Lyt2 |
| Location | Cell membrane |
| Accession | P01731 |
| Clone Number | 2.43 |
| Antibody Type | Recombinant mAb |
| Isotype | Rat IgG2b, κ |
| Application | WB, FCM, in vivo CD8+ T cell depletion |
| Reactivity | Ms |
| Purification | Protein G |
| Concentration | Lot specific* (generally 5 to 10 mg/ml)* |
| Purity | >95% |
| Endotoxin | <2EU/mg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, containing no preservative |
| Stability & Storage |
2 to 8 °C for 2 weeks under sterile conditions; -20 °C for 3 months under sterile conditions; -80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.
|
Dilution
| application | dilution | species |
| WB | 1:50 | |
| FCM | 1:50000 |
Background
CD8a, also known as CD8α, is a molecular marker on the surface of T lymphocytes. It belongs to the family of leukocyte differentiation antigens and is primarily expressed on the surface of cytotoxic T lymphocytes (CTLs). CD8a plays a crucial role in immune responses related to cytotoxicity and local inflammation. It has the ability to bind to MHC class I molecules. The expression level of CD8 molecules undergoes changes during various stages of T cell differentiation, development, and in response to T cell stimuli. Additionally, CD8a is closely associated with autoimmune monitoring, humoral immune responses, and transplant reactions. For instance, CD8a can serve as a monitoring indicator for certain autoimmune diseases and plays a significant role in immunotherapy for anti-tumor and anti-viral treatments.
Picture
Picture
Western Blot
FC
Flow cytometric analysis of C57BL/6 mouse splenocytes labeling CD8α at 1/50000 (0.01 μg) dilution / (Right panel) compared with a Rat IgG, Isotype Control / (Left panel). Goat Anti - Rat IgG Alexa Fluor® 488 was used as the secondary antibody. Then cells were stained with CD3 - Alexa Fluor® 647 separately. Gated on total viable cells.
