Flow cytometric analysis of Mouse Perforin expression in BALB/c mouse splenocytes. BALB/c mouse splenocytes were fixed and permeabilized with Foxp3 / Transcription Factor Staining Buffer Set. The cells were then washed and stained in Perm/Wash™ Buffer with FITC Rat Anti-Mouse CD49b antibody and with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control or SDT Alexa Fluor® 647 Rat Anti-Mouse Perforin Antibody at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse Perforin Antibody (S-R644)
Alexa Fluor® 647 Rat Anti-Mouse Perforin Antibody (S-R644)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Perforin |
| Synonyms | Perforin-1; P1; Cytolysin; Lymphocyte pore-forming protein; Pfp; Prf1 |
| Location | Secreted, Cell membrane |
| Accession | P10820 |
| Clone Number | S-R644 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | BALB/c mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Ms |
Background
Perforin is a crucial protein involved in the immune response, primarily produced by cytotoxic T lymphocytes and natural killer cells. It functions by forming pores in the membranes of target cells, such as virus-infected or cancerous cells. Once released from immune cells, perforin molecules aggregate and insert into the target cell membrane, creating channels that disrupt the cell's osmotic balance, leading to cell lysis and death. This mechanism is essential for eliminating harmful cells and maintaining the body's overall health.
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