Flow cytometric analysis of IL-8 expression in stimulated Human PBMC (human peripheral blood mononuclear cells). Human PBMC were cultured 6 hr with 10ng/ml lipopolysaccharide in the presence of 1μg/ml BFA. The PBMC were harvested and fixed with 4% PFA and permeabilized with Intracellular Fixation & Permeabilization Buffer Set. The cells were then stained with Brilliant Violet 510™ Mouse Anti-Human CD14 and either Mouse IgG2b Isotype Control or SDT Alexa Fluor® 647 Mouse Anti-Human IL-8 Antibody at 1.25μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Mouse Anti-Human IL-8 Antibody (S-2875)
Alexa Fluor® 647 Mouse Anti-Human IL-8 Antibody (S-2875)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | IL-8 |
| Synonyms | Interleukin-8; IL-8; C-X-C motif chemokine 8; Chemokine (C-X-C motif) ligand 8; Emoctakin; Granulocyte chemotactic protein 1 (GCP-1); Monocyte-derived neutrophil chemotactic factor (MDNCF); IL8; CXCL8 |
| Location | Secreted |
| Accession | P10145 |
| Clone Number | S-2875 |
| Antibody Type | Mouse mAb |
| Isotype | IgG2b |
| Application | ICFCM |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICFCM | 1.25μl per million cells in 100μl volume | Hu |
Background
Interleukin-8 (IL-8), also known as CXCL8, is a small 8–10 kDa chemokine produced by monocytes, macrophages, endothelial cells, and epithelial cells in response to inflammatory stimuli such as TNF-α, IL-1β, or bacterial LPS; it acts primarily through the G-protein-coupled receptors CXCR1 and CXCR2 on neutrophils to induce rapid chemotaxis, degranulation, and respiratory burst, thereby orchestrating acute inflammatory responses, angiogenesis, and tumor microenvironment remodeling, while dysregulated IL-8 expression is implicated in chronic inflammatory diseases, COPD, atherosclerosis, cancer progression, and metastasis.
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