In the experimental design, 4 μg RNA was incubated with 0 or 2.5 ng RNase A and 1, 0.5, 0.25, 0.125 or 0 μL RNase Inhibitor in 20 μL reaction system, incubated for 15 minutes at 37°C. The reaction was analyzed by 1% agarose gel electrophoresis. The figure is the effect of RNase Inhibitor on RNase A enzyme activity.
Lane 1 Negative Control (Only negative controls with no RNase A were added);
Lane 2 UA-Murine RNase Inhibitor 0 μL;
Lane 3 UA-Murine RNase Inhibitor 0.125 μL;
Lane 4 UA-Murine RNase Inhibitor 0.25 μL;
Lane 5 UA-Murine RNase Inhibitor 0.5 μL;
Lane 6 UA-Murine RNase Inhibitor 1 μL;
Murine RNase Inhibitor
Murine RNase Inhibitor
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$40 USD
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$40 USD
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Product Details
Product Details
Product Specification
| Species | Rat |
| Synonyms | Ribonuclease inhibitor |
| Expression System | E.coli |
| Molecular Weight | 50 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Storage Buffer | 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃) |
| Stability & Storage | Store at -25 ~ -15℃ for 2 years |
| Reference | [1] Wanhua, Guo, and et al. High level soluble production of functional ribonuclease inhibitor in Escherichia coli by fusing it to soluble partners[J]. Protein Expression & Purification, 2011. |
Background
Murine RNase inhibitor is expressed in soluble form in E. coli and capable of inhibiting a wide range of RNases (RNase A, B, C). Murine RNase inhibitor was tested by RT-PCR and RT-qPCR, and was compatible with various commercial reverse transcriptase’s and various DNA polymerases. Compared with human RNase inhibitor, murine RNase inhibitor does not contain two cysteines that are very sensitive to oxidation, so it has higher antioxidant activity and is more suitable for high DTT sensitive experiments (such as qPCR).
Components
Storage Solution: 40 U/µL Murine RNase Inhibitor, 20 mM HEPES-KOH, 50 mM KCl, 8 mM DTT, 50% Glycerol (pH 7.6 @ 25℃)
Protocol
Add inhibitor to achieve final concentration of 1 U/μL in your reaction.
During assembly of a reaction, RNase Inhibitor should be added before other components that are a possible source of RNase contamination.
Guidelines
Please avoid repeated freeze-thaw cycles.
Unit Definition
One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A.
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Bioactivity
SDS-PAGE
1 μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
