| Species | Streptococcus pyogenes |
| Synonyms | IgG endopeptidase |
| Amino Acid Sequence | N.A |
| Expression System | E.coli |
| Molecular Weight | 36kDa |
| Purity | >95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5 |
| Reconstitution | N.A |
| Stability & Storage |
12 months from date of receipt, -20 to -70 °C as supplied; |
| Reference |
1.Shi S, Mcleod B, Magnelli P, et al. The Utility of IdeZ Protease in Glycan Profiling of Therapeutic Antibodies[J]. Glycobiology, 2016(12):26. 2.Cheng-Wei H, Jie P, Yu-Qing O, et al. Characteristics of IgG degradation enzyme IdeZ in Streptococcus equi ssp. zooepidimicus[J]. Chinese Journal of Preventive Veterinary Medicine, 2018. |
IdeZ Protease secreted by the Streptococcus equi subspecies zooepidemicus. This product is an engineered recombinant protease over expressed in Escherichia coli. It can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and produce F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.
1. Add the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.
2. Add IdeZ Protease to the reaction system:
• Add 1 unit of IdeZ Protease per 1µg of IgG to be digested.
• For example, add 5µl (200 units) of reconstituted IdeZ to digest 200µg of IgG.
3. Incubate sample at 37°C for 30–60 minutes
Results of 5μg rabbit IgG digestion separated under different quantity of IdeZ protease, The reaction was incubated for 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate)
M, marker:
lane 1 rabbit IgG;
lane 2 rabbit IgG add 5 units IdeZ;
lane 3 rabbit IgG add 10 units IdeZ;
lane 4 rabbit IgG add 33units IdeZ;
lane 5 rabbit IgG add 50 units IdeZ
M: marker; Complement IdeZ protease, 2μg on SDS-PAGE
under Non-reducing and reducing condition. The purity is greater than 95%
