IdeS Protease

Catalog Number: UA070027 Reactivity: Other Conjugation: Unconjugated Brand: UA BIOSCIENCE

Price:

$330 USD

Size: 2000U 5000U

Quantity:

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Product Details

Product Specification

Species	Streptococcus pyogenes
Synonyms	IdeS
Amino Acid Sequence	N.A
Expression System	E.coli
Molecular Weight	37.7kDa
Purity	>95% by SDS-PAGE
Conjugation	Unconjugated
Tag	His Tag
Physical Appearance	Liquid
Storage Buffer	PBS pH 6.6, 50% glycerol
Reconstitution	N.A
Stability & Storage	12 months from date of receipt, -20 to -70 °C as supplied; 1 week, 2 to 8 °C under sterile conditions; Please avoid repeated freeze-thaw cycles.
Reference	1.Vincents B, Von Pawel-Rammingen U, Bj?Rck L ,et al.Enzymatic characterization of the streptococcal endopeptidase, IdeS, reveals that it is a cysteine protease with strict specificity for IgG cleavage due to exosite binding.[J].Biochemistry, 2004, 43(49):15540-15549. 2.Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615.

Background

IdeS Protease is a cysteine hydrolase secreted by the human pathogen Streptococcuspyogenes. This product use E. coli recombinant expression, high purity, has good enzyme digestion activity, can specifically identify IgG, and perform enzyme digestion at specific sites in the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2 fragments and Fc fragments, which can identify human and other animal IgG, such as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be used for structural characterization analysis of antibodies and fusion protein drugs.

Protocol

1. Add the desired amount of 5mg IgG in digestion buffer or other compatible buffer*.

2. Add IdeS Protease to the reaction system:

• Add 1 unit of IdeS Protease per 1µg of IgG to be digested.

• For example, add 5µl (200 units) of reconstituted IdeS to digest 200µg of IgG.

3. Incubate sample at 37°C for 30–60 minutes

Unit Definition

One unit will cleave ≥95% of 1µg of recombinant monoclonal IgG in 30 minutes at 37°C.

Picture

Bioactivity

Results of 5μg rabbit IgG digestion separated under different quantity of IdeS, The reaction was incubated for8 30 minutes at 37°C using the recommended digestion buffer (50mM sodium phosphate).

M, marker;

lane 1 rabbit IgG;

lane 2 rabbit IgG add 5 units IdeS;

lane 3 rabbit IgG add 6.25 units IdeS;

lane 4 rabbit IgG add 8.3 units IdeS;

lane 5 rabbit IgG add 12.5 units IdeS;

lane 6 rabbit IgG add 25 units IdeS

SDS-PAGE

M: marker ; Complement IdeS protease，2μg on SDS-PAGE under Non-reducing and reducing condition . The purity is greater than 95%.

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IdeS Protease

IdeS Protease