Standard curve
Example of Mouse TNF-α standard curve in Assay Diluent #1.
Mouse TNF-α OneStep ELISA Kit
Mouse TNF-α OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | TNF-α |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Ms |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 3.8%； Inter-assay: 5.8% |
| Sample type | Cell culture supernatant, Serum, Plasm |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.095 ng/ml |
| Range | 0.156 ng/mL – 10 ng/mL |
| Recovery | Cell culture supernatant: 87% Serum: 106% Plasm: 105% |
| Assay time | 60 minutes |
| Species reactivity | Mouse |
| CROSS REACTIVITY | / |
| INTERFERENCE | / |
| NIBSC convert | / |
Background
Tumor necrosis factor α (TNF alpha) is an effective pro-inflammatory cytokine, which can kill and inhibit tumor cells. It is mainly produced by activated macrophages and can also be secreted by other types of cells, such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neuronal tissues. The members of TNF alpha family exert their cellular effect through two distinct surface receptors of the TNF receptor family, TNFR1and TNFR2. The pleiotropic biological effects of TNF can be attributed to its ability to simultaneously activate multiple signaling pathways in cells. TNF-induced NF-κB activation promotes the growth, invasion and metastasis of cancer cells.
Picture
Picture
ELISA
Linearity
The concentrations of TNF-α were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: RAW264.7 mouse monocyte/macrophage cells stimulated with 5ug/ml LPS and 100ng/mL rmIL-10 for 7days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.19 ng/mL in stimulated RAW264.7 supernatant.
Protocol Diagram
