Standard curve
Example of Human TNF-α standard curve in Assay Diluent #1.
Human TNF-α OneStep ELISA Kit
Human TNF-α OneStep ELISA Kit
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Product Details
Product Details
Product Specification
| Antigen | TNF-α |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision |
Intra-assay: 6.8%； Inter-assay: 8.9% |
| Sample type | Cell culture supernatant, Serum, Plasm |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 8.763 pg/ml |
| Range | 21.9 pg/mL – 1404.6 pg/mL |
| Recovery | Cell culture supernatant: 95% Serum: 103% Plasm: 108% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| NIBSC convert | The dose response curve of the International Standard (12/154) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (12/154) units, use the following equation: NIBSC (12/154) approximate value (IU/mL) = 0.43 x OneStep Human TNF-α value (pg/mL). |
Background
TNF alpha is produced by various types of cells including macrophages, monocytes, neutrophils, T cells, and NK-cells. TNF-alpha is critical for normal immune response, abnormal secretion TNF alpha activates synovial fibroblasts, keratinocytes, osteoclasts, induces rheumatoid arthritis, inflammatory bowel disease, psoriatic arthritis (PsA), and noninfectious uveitis (NIU). TNF alpha positively regulates endogenous TNF-α expression levels independently of Pgp efflux activity, induces IHF cells proliferation. TNF alpha in tissues may promote cancer growth, invasion, and metastasis. Besides, TNF alpha stimulates NF-κB pathway via TNFR2 and anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse. TNF alpha as a proneurogenic factor activates the SAPK/JNK pathway and can facilitate neuronal replacement and brain repair in response to brain injury.
Picture
Picture
ELISA
Linearity
The concentrations of TNF-α were measured and interpolated from the target standard curves and corrected for sample dilution.
Sample is undiluted samples are as follows: human PBMC cells stimulated with 10ng/ml PMA and 500ng/mL lonomycin Calcium Salt for 24 hours (25%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 13.99 ng/mL in stimulated human PBMC supernatant.
Protocol Diagram
