Standard curve
Example of Human IFN-γ standard curve in Assay Diluent #10.
Human IFN-γ OneStep ELISA Kit
Human IFN-γ OneStep ELISA Kit
Price:
Regular price
$500 USD
Regular price
Sale price
$500 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Antigen | IFN-γ |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 2 to 8 °C as supplied. |
Kit
| Precision | 板内变异系数 (Intra-assay): 5.0%； 板间变异系数 (Inter-assay): 6.2% |
| Sample type | Cell culture supernatant, Serum, Plasm |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 0.6 pg/ml |
| Range | 3.4 pg/mL – 217.4 pg/mL |
| Recovery | Cell culture supernatant 86% Serum 116% Plasm 90% |
| Assay time | 60 minutes |
| Species reactivity | Human |
| NIBSC convert |
The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).
|
Background
Interferon gamma (IFN-γ) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. A non-IgE-mediated anaphylactic reaction and severe bronchospasm have been reported once after the first injection of interferon gamma. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins. Production of IFN-γ is largely restricted to activated CD4+ TH1 T cells, CD8+ T cells, and natural killer cells. One of the most important consequences of IFN-γ secretion is the activation of macrophages. In addition, IFN-γ plays a central role in inflammatory responses by activating endothelial cells, promoting TH1 cell development and cellular immune responses, and up-regulation of major histocompatability complex protein expression on antigen-presenting cells.
Picture
Picture
ELISA
Standard curve
Example of NIBSC (87/586) standard curve in Assay Diluent #1. Human IFN-γ standard were calibrated by NIBSC (87/586) with a calibration factor (CF) of 1.0. Human IFN-γ standard concentration = NIBSC (87/586)/CF
Linearity
The concentrations of IFNγ were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (1%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 31,555 pg/mL in stimulated human PBMC supernatant.
#2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 112 pg/mL in unstimulated human PBMC supernatant.
To assess the linearity of the assay, three samples were spiked with high concentrations of Human IFNγ in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Leading Competitor comparison
Protocol Diagram
